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8. | | OKINO, C. H.; GIGLIOTI, R.; Silva, P. C.; OLIVEIRA, H. N. de; OLIVEIRA, M. C. de S. Comparative evaluation of DNA extraction kit, matrix sample and qPCR assays for bovine babesiosis monitoring. Molecular Biology Reports, v. 45, n. 6, p. 2671-2680, 2018. Biblioteca(s): Embrapa Pecuária Sudeste. |
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11. | | OLIVEIRA, M. C. de S.; CHAGAS, A. C. de S.; FORIM, M.; SILVA, M. F.; GIGLIOTI, R.; CARVALHO, C. O. Ação de extrato de Neem sobre Rhipicephalus ( Boophilus micropulus) com azadirachtina - A quantificada por HPLC. In: REUNION ASOCIACION LATINOAMERICANA DE PRODUCCION ANIMAL, 20.; REUNION ASOCIACION PERUANA DE PRODUCCION ANINAL, 30.; CONGRESSO INTERNACIONAL DE GANDEIRA DE DOBLE PROPOSTO, 5., 2007, Cuzco, Perú. Annales... Cuzco: ALPA: APPA, 2007. 1 CD-ROM. Resumo na seguinte fonte: Archivos Latinoamericano de Produccion Animal, v. 15, (supl.), p. 489, 2007 Biblioteca(s): Embrapa Pecuária Sudeste. |
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12. | | FUNES-HUACCA, M.E.; GIGLIOTI, R.; NÉO, T. A.; CARRILHO, E.; BRITO, L. G.; OLIVEIRA, M. C. de S. ANÁLISE DO PERFIL PROTÉICO DOS PRODUTOS DE EXCREÇÃO/SECREÇÃO DE LARVAS DE Cochliomyia hominivorax. In: SIMPÓSIO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA PECUÁRIA SUDESTE, 2006, São Carlos, SP. Anais... São Carlos: Embrapa Pecuária Sudeste, 2006. Biblioteca(s): Embrapa Pecuária Sudeste. |
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13. | | BASSETTO, C. C.; OKINO, C. H.; GIGLIOTI, R.; OLIVEIRA, H. N. de; OLIVEIRA, M. C. de S. Levels of antibodies against babesia bigemina and babesia bovis in cows and calves by elisa using recombinant antigens. In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 20., 2018, Londrina, PR. Anais...Londrina, PR: CBPV, 2018. p. 255 Biblioteca(s): Embrapa Pecuária Sudeste. |
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15. | | GIGLIOTI, R.; OKINO, C. H.; BASSETTO, C. C.; SILVA, P. C.; OLIVEIRA, H. N. de; OLIVEIRA, M. C. de S. New Loop-mediated isothermal amplification (lamp) method for the rapid identification of anaplasma marginale. In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 20., 2018, Londrina, PR. Anais...Londrina, PR: CBPV, 2018. p. 259 Biblioteca(s): Embrapa Pecuária Sudeste. |
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16. | | BRITO, L. G.; BARBIERI, F. da S.; GIGLIOTI, R.; SOUZA, G. C. R.; BENITEZ, F.; OLIVEIRA, M. C. de S. Babesia bovis infection in catlle from Rondonia, Brazil. In: WORLD BUATRICS CONGRESS, 26., 2010, Santiago. Abstracts...Santiago: BUATRICS, 2010. Biblioteca(s): Embrapa Pecuária Sudeste; Embrapa Rondônia. |
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17. | | PORTILHO, A. I.; GIGLIOTI, R.; OLIVEIRA, H. N. de; MARCONDES, C. R.; OKINO, C. H.; OLIVEIRA, M. C. de S. Associação entre os níveis de infecção por Babesia bovis e Babesia bigemina em amostras de sangue e carrapatos Rhipicephalus microplus colhidos em bovinos da raça Canchim. In: JORNADA CIENTÍFICA DA EMBRAPA SÃO CARLOS, 9., 2017, São Carlos, SP. Anais... São Carlos, SP: Embrapa Pecuária Sudeste; Embrapa Instrumentação, 2017. p. 37. (Embrapa Pecuária Sudeste. Documentos, 126). Editores técnicos: Alexandre Berndt, Ana Rita Araujo Nogueira, Bianca Baccili Zanotto Vigna, Juliana Gonçalves Costa, Lea Chapaval, Manuel Antonio Chagas Jacinto, Patricia Menezes Santos. Biblioteca(s): Embrapa Pecuária Sudeste. |
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18. | | CHAGAS, A. C. de S.; OLIVEIRA, M. C. de S.; FORIM, M. R.; GIGLIOTI, R.; MIGLIATO, M. A. T. Avaliação do óleo de Neem com teor padronizado de azadirachtina - A sobre as larvas de Rhipicephalus Boophilus microplus. In: CONGRESSO BRASILEIRA DE VETERINÁRIA, 34., 2007, Santos. Resumos... São Paulo, Sociedade Paulista de Medicina Veterinária, 2007. 1 CD-ROM. Biblioteca(s): Embrapa Pecuária Sudeste. |
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19. | | GIGLIOTI, R.; OLIVEIRA, H. N.; BILHASSI, T. B.; CAVANI, L.; MARCONDES, C. R.; OLIVEIRA, M. C. de S. Repetibilidade e associação entre os níveis de infecção por hemoparasitas e infestação por carrapatos em bovinos da raça Canchim. In: SIMPÓSIO BRASILEIRO DE MELHORAMENTO ANIMAL, 12., 2017, Ribeirão Preto, SP. Anais... Sertãozinho: SBMA, 2017. p. 1-3. Biblioteca(s): Embrapa Pecuária Sudeste. |
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20. | | GIGLIOTI, R.; BILHASSI, T. B.; MACHADO, R. Z.; OLIVEIRA, H. N. de; OLIVEIRA, M. C. de S. Repetibilidade e associação entre técnicas moleculares e sorológicas quantitativas na detecção de babesia bovis em bovinos de corte criados no estado de São Paulo, Brasil. In: CONGRESO DE LA ASOCIACIÓN LATINO AMERICANA, 24.; CONGRESO DE LA SOCIED CHILENA DE PRODUCIÓN ANIMAL, 40., 2015, Puerto Varas. Anales... Puerto Varas: ALPA, 2015. 1 CD-ROM Biblioteca(s): Embrapa Pecuária Sudeste. |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Pecuária Sudeste; Embrapa Rondônia. |
Data corrente: |
11/11/2010 |
Data da última atualização: |
14/12/2010 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
BRITO, L. G.; BARBIERI, F. da S.; GIGLIOTI, R.; SOUZA, G. C. R.; BENITEZ, F.; OLIVEIRA, M. C. de S. |
Afiliação: |
LUCIANA GATTO BRITO, CPAF-RO; FABIO DA SILVA BARBIERI, CPAF-RO; RODRIGO GIGLIOTI, UFSCar/SÃO CARLOS, SP; GISLAINE CRISTINA RODRIGUES DE SOUZA, UFRo/PORTO VELHO; FABIANO BENITEZ, IDARON/PORTO VELHO; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE. |
Título: |
Babesia bovis infection in catlle from Rondonia, Brazil. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
In: WORLD BUATRICS CONGRESS, 26., 2010, Santiago. Abstracts...Santiago: BUATRICS, 2010. |
Idioma: |
Português |
Conteúdo: |
In Latin America, bovine babesiosis is due to Babesia bovis and Babesia bigemina, which are exclusively transmitted by the tick Rhipicephalus (Boophilus) micro plus. Most of the Brazilian territory is endemic for R. micro plus, condition also present in Rondonia State. Objective: The present study provides the first epidemiological data regarding infection by Babesia bovis in cattle reared in south- western Brazilian Amazonia. Material and methods: The study was developed with blood clots donated by Animal Health Services of Rondonia and Acre states. All evaluated cattle with 4-12 months of age were from eight microregions of Rondonia: Alvorada do Oeste, Cacoal, Guajara-Mirim,and Ji-Parana. Based on an estimated prevalence of cattle 70.0% positive for B. bovis, was found for a sampling of 45 samples by region. One simple procedure was adapted for the extraction of DNA from blood clots collected in four microregions of Rondonia State. PCR method was used to assess the frequency of B. bovis infections in 4 to12-month-old cattle.The cattle infection was investigated by nested polymerase chain reaction (nPCR) using the specifics primers for B. bovis. Results and discussion: The DNA amplifications revealed that the mean frequency of B. bovisinfection was 94.75% (286/272) in samples from Rondonia. The high frequency of B. bovis infections in 4 to 12-month-old cattle indicate a situation of enzootic stability in the studied areas and are comparable to those detected by immunodiagnosis in different endemic regions in Brazil. The DNA extraction of clotted blood method can be used for epidemiological studies on bovine babesiosis and other bovine hemoparasites. Conclusions: The results obtained in Rondonia state not differ from other Brazilian regions, where B. bovis infection is also high and independent of others factors like age and racial traits. However, similar epidemiological patterns are expected in other regions between the parallels 320 South and 320 North, where the main vector of B. bovis, the R. micro plus tick. MenosIn Latin America, bovine babesiosis is due to Babesia bovis and Babesia bigemina, which are exclusively transmitted by the tick Rhipicephalus (Boophilus) micro plus. Most of the Brazilian territory is endemic for R. micro plus, condition also present in Rondonia State. Objective: The present study provides the first epidemiological data regarding infection by Babesia bovis in cattle reared in south- western Brazilian Amazonia. Material and methods: The study was developed with blood clots donated by Animal Health Services of Rondonia and Acre states. All evaluated cattle with 4-12 months of age were from eight microregions of Rondonia: Alvorada do Oeste, Cacoal, Guajara-Mirim,and Ji-Parana. Based on an estimated prevalence of cattle 70.0% positive for B. bovis, was found for a sampling of 45 samples by region. One simple procedure was adapted for the extraction of DNA from blood clots collected in four microregions of Rondonia State. PCR method was used to assess the frequency of B. bovis infections in 4 to12-month-old cattle.The cattle infection was investigated by nested polymerase chain reaction (nPCR) using the specifics primers for B. bovis. Results and discussion: The DNA amplifications revealed that the mean frequency of B. bovisinfection was 94.75% (286/272) in samples from Rondonia. The high frequency of B. bovis infections in 4 to 12-month-old cattle indicate a situation of enzootic stability in the studied areas and are comparable to those detected by immunodiagno... Mostrar Tudo |
Palavras-Chave: |
Rondonia. |
Thesagro: |
Babesia Bovis. |
Thesaurus NAL: |
beef cattle; infection. |
Categoria do assunto: |
H Saúde e Patologia L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02687nam a2200217 a 4500 001 1869876 005 2010-12-14 008 2010 bl uuuu u00u1 u #d 100 1 $aBRITO, L. G. 245 $aBabesia bovis infection in catlle from Rondonia, Brazil.$h[electronic resource] 260 $aIn: WORLD BUATRICS CONGRESS, 26., 2010, Santiago. Abstracts...Santiago: BUATRICS$c2010 520 $aIn Latin America, bovine babesiosis is due to Babesia bovis and Babesia bigemina, which are exclusively transmitted by the tick Rhipicephalus (Boophilus) micro plus. Most of the Brazilian territory is endemic for R. micro plus, condition also present in Rondonia State. Objective: The present study provides the first epidemiological data regarding infection by Babesia bovis in cattle reared in south- western Brazilian Amazonia. Material and methods: The study was developed with blood clots donated by Animal Health Services of Rondonia and Acre states. All evaluated cattle with 4-12 months of age were from eight microregions of Rondonia: Alvorada do Oeste, Cacoal, Guajara-Mirim,and Ji-Parana. Based on an estimated prevalence of cattle 70.0% positive for B. bovis, was found for a sampling of 45 samples by region. One simple procedure was adapted for the extraction of DNA from blood clots collected in four microregions of Rondonia State. PCR method was used to assess the frequency of B. bovis infections in 4 to12-month-old cattle.The cattle infection was investigated by nested polymerase chain reaction (nPCR) using the specifics primers for B. bovis. Results and discussion: The DNA amplifications revealed that the mean frequency of B. bovisinfection was 94.75% (286/272) in samples from Rondonia. The high frequency of B. bovis infections in 4 to 12-month-old cattle indicate a situation of enzootic stability in the studied areas and are comparable to those detected by immunodiagnosis in different endemic regions in Brazil. The DNA extraction of clotted blood method can be used for epidemiological studies on bovine babesiosis and other bovine hemoparasites. Conclusions: The results obtained in Rondonia state not differ from other Brazilian regions, where B. bovis infection is also high and independent of others factors like age and racial traits. However, similar epidemiological patterns are expected in other regions between the parallels 320 South and 320 North, where the main vector of B. bovis, the R. micro plus tick. 650 $abeef cattle 650 $ainfection 650 $aBabesia Bovis 653 $aRondonia 700 1 $aBARBIERI, F. da S. 700 1 $aGIGLIOTI, R. 700 1 $aSOUZA, G. C. R. 700 1 $aBENITEZ, F. 700 1 $aOLIVEIRA, M. C. de S.
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